| 质粒名称: | pLenti CMV GFP Hygro (656-4) |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 17446 |
| 存储实验室: | Eric Campeau, Paul Kaufman |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 空载体大小: | 8591bp |
| 完整质粒大小: | 9320 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Hygromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | enhanced GFP/GFP |
| 插入大小: | 719bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | Sal I (未破坏) |
| 5’测序引物 | CMV-F |
|
文献引用方法 |
|
| 材料和方法部分: |
pLenti CMV GFP Hygro (656-4) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17446 ; http://n2t.net/addgene:17446 ; RRID:Addgene_17446)
For viral preps, please replace (Addgene plasmid # 17446) in the above sentence with: (Addgene viral prep # 17446-LV) or (Addgene viral prep # 17446-LVC)
|
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pLenti CMV GFP Hygro (656-4) 9320 bp ds-DNA circular SYN 26-MAY-2021
DEFINITION 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro.
ACCESSION .
VERSION .
KEYWORDS pLenti CMV GFP Hygro (656-4)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9320)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A versatile viral system for expression and depletion of proteins in
mammalian cells.
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
REFERENCE 2 (bases 1 to 9320)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 26, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9320
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 94..111
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 228..249
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 264..294
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 302..318
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 307..329
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 326..342
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 326..342
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 361..381
/label=T3
/note="T3 promoter, forward primer"
promoter 363..381
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 409..635
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 636..816
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 863..988
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1481..1714
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1899..1943
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2210..2326
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2349..2652
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2653..2856
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2807..2827
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2853..2877
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2946..2955
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2952..3671
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2952..3668
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2997..3018)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3258..3277)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3605..3626
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 3687..4275
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3740..3760)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4158..4169)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 4331..4830
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(4366..4389)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 4763..4782
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 4848..5873
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
LTR 6247..6480
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6552..6673
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6589..6608)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6643..6662
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6713..6848
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6775..6794
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6868..6887)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6869..6887)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6897..6914)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6897..6913)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6906..6928)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 7055..7510
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7142..7161)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7352..7373
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 7536..7640
/gene="bla"
/label=AmpR promoter
CDS 7641..8501
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7859..7878)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8672..9260
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9161..9180
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
2341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
2401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
2461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
2521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
2581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
2641 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
2701 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
2761 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
2821 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaggatccac
2941 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
3001 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
3061 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
3121 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
3181 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
3241 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
3301 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
3361 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
3421 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
3481 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
3541 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
3601 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
3661 tgtacaagta aagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
3721 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
3781 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
3841 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
3901 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
3961 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
4021 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
4081 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
4141 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
4201 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
4261 gggccgcctc cccgcctgga attctgcaga tatccggact agtgatctct cgaggttaac
4321 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
4381 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
4441 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
4501 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
4561 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
4621 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
4681 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
4741 cgaaggccct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
4801 ttctcctctt cctcatctcc gggcctttcg acctgcatcc cgccaccatg aaaaagcctg
4861 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
4921 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
4981 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
5041 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
5101 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
5161 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
5221 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
5281 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
5341 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
5401 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
5461 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
5521 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
5581 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
5641 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
5701 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
5761 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
5821 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat
5881 gccgaccgaa caagagctga tttcgagaac gcctcagcca gcaactcgcg cgagcctagc
5941 aaggcaaatg cgagagaacg gccttacgct tggtggcaca gttctcgtcc acagttcgct
6001 aagctcgctc ggctgggtcg cgggagggcc ggtcgcagtg attcaggccc ttctggattg
6061 tgttggtccc cagggcacga ttgtcatgcc cacgcactcg ggtgatctga ctgatcccgc
6121 agattggaga tcgccgcccg tgcctgccga ttgggtgcag atccgtcgag ggcccggtac
6181 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
6241 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
6301 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
6361 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
6421 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
6481 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
6541 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
6601 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
6661 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
6721 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
6781 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
6841 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
6901 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
6961 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
7021 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
7081 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
7141 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
7201 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
7261 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
7321 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
7381 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
7441 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
7501 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
7561 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
7621 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
7681 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
7741 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
7801 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
7861 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
7921 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
7981 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
8041 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
8101 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
8161 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
8221 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
8281 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
8341 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
8401 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
8461 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
8521 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
8581 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
8641 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
8701 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
8761 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
8821 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
8881 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
8941 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
9001 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
9061 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
9121 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
9181 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
9241 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
9301 gctggccttt tgctcacatg
//
